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 Enhanced Culturing Techniques Improve Detection of Clinically Relevant Microorganisms.

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Andi2016

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Join date : 2016-01-07

PostSubject: Enhanced Culturing Techniques Improve Detection of Clinically Relevant Microorganisms.   Tue Jul 26, 2016 12:34 pm

This 2016 US study used enhanced quantitative urine culture (EQUC) on 150 adult uro-gynecology patient-participants. They detected live microorganisms in the vast majority of specimens reported as 'no growth' by standard urine culturing. Overall, they found 67 percent of uropathogens were missed by standard culturing, and 50 percent in those with severe urinary symptoms.

The EQUC uses 100 times more urine than standard culturing techniques. The technique uses 100 μl of urine plated onto blood (blood agar plate [BAP]), colistin-nalidixic acid (CNA), and MacConkey agars in 5% CO2 for 48 h.

"This streamlined EQUC protocol achieved 84% uropathogen detection relative to 33% detection by standard urine culture. The streamlined EQUC protocol improves detection of uropathogens that are likely relevant for symptomatic women, giving clinicians the opportunity to receive additional information not currently reported using standard urine culture techniques."

http://www.ncbi.nlm.nih.gov/pubmed/26962083
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